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    Structured Review

    Greiner Bio pipette tips
    Pipette Tips, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/universal+pipette+tips/Pipette+Tips+10+-+200+%CE%9Cl+Universal/pmc12706807-32-0-3
    Average 93 stars, based on 34 article reviews
    pipette tips - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Sequencing:

    Article Title: Generation of Recombinant SARS‐CoV‐2 Using a Bacterial Artificial Chromosome
    Article Snippet: The SARS‐CoV‐2 USA‐WA1/2020 sequence to generate the rSARS‐CoV‐2 is available from Genbank (accession no. MN985325) Post‐infection medium for Vero E6 cells: Dulbecco's Modified Eagle Medium (DMEM; Corning, cat. no. 15‐013‐CV) containing 2% fetal bovine serum (FBS; Avantor Seradigm, cat. no. 1500‐500) and 1× penicillin‐streptomycin‐ l ‐glutamine (PSG; Corning, cat. no. 30‐009‐CI) Phosphate‐buffered saline (PBS; see ) 10% neutral buffered formalin solution (Sigma‐Aldrich, cat. no. HT501128–4 liters) Permeabilization solution: 0.5% (v/v) Triton X‐100 (Sigma‐Aldrich, cat. no. X100−500 ML) in 1× PBS (see ) Blocking solution: 2.5% bovine serum albumin (BSA; Sigma‐Aldrich, cat. no. A9647) in 1× PBS (see ) Mouse anti‐SARS NP MAb 1C7 cross‐reactive with SARS‐CoV‐2 (Millipore Sigma, cat. no. ZMS1075) was generated at the Center for Therapeutic Antibody Development at the Icahn School of Medicine at Mount Sinai (ISMMS) and kindly provided by Dr. Thomas Moran Polyclonal rabbit anti‐mouse FITC (Dako, cat. no. F0261) 4′,6′−diamidino‐2‐phenylindole (DAPI; Research Organics, cat. no. 2039D) DMEM/F12 agar medium (see ) Vectastain ABC‐HRP kit, peroxidase, mouse IgG (Vector laboratories, cat. no. P‐4002) Biotinylated anti‐mouse secondary antibody (from Vectastain ABC‐HRP kit) DAB substrate kit, peroxidase (HRP), with nickel (Vector Laboratories, cat. no. SK‐4100) Avicel PH‐101 (Sigma‐Aldrich, cat. no. 11365‐1KG) Tissue culture plates: 6‐, 12‐, and 96‐well cell culture plates (Greiner Bio‐One, cat. no. 657160, 665180, and 655180, respectively) Polypropylene sterile conical tubes, 15‐ and 50‐ml (Greiner Bio‐One, cat. no. 188261 and 227270, respectively) Serological pipette, 5, 10, and 25 ml (Greiner Bio‐One, cat. no. 606180, 607180, and 760180, respectively) Universal pipette tips, 20, 200, and 1000 μl (VWR, cat. no. 76322‐134, 76322‐150, and 16466‐008, respectively) Incubator (PHCbi, model cat. no. MCO‐170AICUVDL) Fluorescence microscope

    Modification:

    Article Title: Generation of Recombinant SARS‐CoV‐2 Using a Bacterial Artificial Chromosome
    Article Snippet: The SARS‐CoV‐2 USA‐WA1/2020 sequence to generate the rSARS‐CoV‐2 is available from Genbank (accession no. MN985325) Post‐infection medium for Vero E6 cells: Dulbecco's Modified Eagle Medium (DMEM; Corning, cat. no. 15‐013‐CV) containing 2% fetal bovine serum (FBS; Avantor Seradigm, cat. no. 1500‐500) and 1× penicillin‐streptomycin‐ l ‐glutamine (PSG; Corning, cat. no. 30‐009‐CI) Phosphate‐buffered saline (PBS; see ) 10% neutral buffered formalin solution (Sigma‐Aldrich, cat. no. HT501128–4 liters) Permeabilization solution: 0.5% (v/v) Triton X‐100 (Sigma‐Aldrich, cat. no. X100−500 ML) in 1× PBS (see ) Blocking solution: 2.5% bovine serum albumin (BSA; Sigma‐Aldrich, cat. no. A9647) in 1× PBS (see ) Mouse anti‐SARS NP MAb 1C7 cross‐reactive with SARS‐CoV‐2 (Millipore Sigma, cat. no. ZMS1075) was generated at the Center for Therapeutic Antibody Development at the Icahn School of Medicine at Mount Sinai (ISMMS) and kindly provided by Dr. Thomas Moran Polyclonal rabbit anti‐mouse FITC (Dako, cat. no. F0261) 4′,6′−diamidino‐2‐phenylindole (DAPI; Research Organics, cat. no. 2039D) DMEM/F12 agar medium (see ) Vectastain ABC‐HRP kit, peroxidase, mouse IgG (Vector laboratories, cat. no. P‐4002) Biotinylated anti‐mouse secondary antibody (from Vectastain ABC‐HRP kit) DAB substrate kit, peroxidase (HRP), with nickel (Vector Laboratories, cat. no. SK‐4100) Avicel PH‐101 (Sigma‐Aldrich, cat. no. 11365‐1KG) Tissue culture plates: 6‐, 12‐, and 96‐well cell culture plates (Greiner Bio‐One, cat. no. 657160, 665180, and 655180, respectively) Polypropylene sterile conical tubes, 15‐ and 50‐ml (Greiner Bio‐One, cat. no. 188261 and 227270, respectively) Serological pipette, 5, 10, and 25 ml (Greiner Bio‐One, cat. no. 606180, 607180, and 760180, respectively) Universal pipette tips, 20, 200, and 1000 μl (VWR, cat. no. 76322‐134, 76322‐150, and 16466‐008, respectively) Incubator (PHCbi, model cat. no. MCO‐170AICUVDL) Fluorescence microscope

    Saline:

    Article Title: Generation of Recombinant SARS‐CoV‐2 Using a Bacterial Artificial Chromosome
    Article Snippet: The SARS‐CoV‐2 USA‐WA1/2020 sequence to generate the rSARS‐CoV‐2 is available from Genbank (accession no. MN985325) Post‐infection medium for Vero E6 cells: Dulbecco's Modified Eagle Medium (DMEM; Corning, cat. no. 15‐013‐CV) containing 2% fetal bovine serum (FBS; Avantor Seradigm, cat. no. 1500‐500) and 1× penicillin‐streptomycin‐ l ‐glutamine (PSG; Corning, cat. no. 30‐009‐CI) Phosphate‐buffered saline (PBS; see ) 10% neutral buffered formalin solution (Sigma‐Aldrich, cat. no. HT501128–4 liters) Permeabilization solution: 0.5% (v/v) Triton X‐100 (Sigma‐Aldrich, cat. no. X100−500 ML) in 1× PBS (see ) Blocking solution: 2.5% bovine serum albumin (BSA; Sigma‐Aldrich, cat. no. A9647) in 1× PBS (see ) Mouse anti‐SARS NP MAb 1C7 cross‐reactive with SARS‐CoV‐2 (Millipore Sigma, cat. no. ZMS1075) was generated at the Center for Therapeutic Antibody Development at the Icahn School of Medicine at Mount Sinai (ISMMS) and kindly provided by Dr. Thomas Moran Polyclonal rabbit anti‐mouse FITC (Dako, cat. no. F0261) 4′,6′−diamidino‐2‐phenylindole (DAPI; Research Organics, cat. no. 2039D) DMEM/F12 agar medium (see ) Vectastain ABC‐HRP kit, peroxidase, mouse IgG (Vector laboratories, cat. no. P‐4002) Biotinylated anti‐mouse secondary antibody (from Vectastain ABC‐HRP kit) DAB substrate kit, peroxidase (HRP), with nickel (Vector Laboratories, cat. no. SK‐4100) Avicel PH‐101 (Sigma‐Aldrich, cat. no. 11365‐1KG) Tissue culture plates: 6‐, 12‐, and 96‐well cell culture plates (Greiner Bio‐One, cat. no. 657160, 665180, and 655180, respectively) Polypropylene sterile conical tubes, 15‐ and 50‐ml (Greiner Bio‐One, cat. no. 188261 and 227270, respectively) Serological pipette, 5, 10, and 25 ml (Greiner Bio‐One, cat. no. 606180, 607180, and 760180, respectively) Universal pipette tips, 20, 200, and 1000 μl (VWR, cat. no. 76322‐134, 76322‐150, and 16466‐008, respectively) Incubator (PHCbi, model cat. no. MCO‐170AICUVDL) Fluorescence microscope

    Blocking Assay:

    Article Title: Generation of Recombinant SARS‐CoV‐2 Using a Bacterial Artificial Chromosome
    Article Snippet: The SARS‐CoV‐2 USA‐WA1/2020 sequence to generate the rSARS‐CoV‐2 is available from Genbank (accession no. MN985325) Post‐infection medium for Vero E6 cells: Dulbecco's Modified Eagle Medium (DMEM; Corning, cat. no. 15‐013‐CV) containing 2% fetal bovine serum (FBS; Avantor Seradigm, cat. no. 1500‐500) and 1× penicillin‐streptomycin‐ l ‐glutamine (PSG; Corning, cat. no. 30‐009‐CI) Phosphate‐buffered saline (PBS; see ) 10% neutral buffered formalin solution (Sigma‐Aldrich, cat. no. HT501128–4 liters) Permeabilization solution: 0.5% (v/v) Triton X‐100 (Sigma‐Aldrich, cat. no. X100−500 ML) in 1× PBS (see ) Blocking solution: 2.5% bovine serum albumin (BSA; Sigma‐Aldrich, cat. no. A9647) in 1× PBS (see ) Mouse anti‐SARS NP MAb 1C7 cross‐reactive with SARS‐CoV‐2 (Millipore Sigma, cat. no. ZMS1075) was generated at the Center for Therapeutic Antibody Development at the Icahn School of Medicine at Mount Sinai (ISMMS) and kindly provided by Dr. Thomas Moran Polyclonal rabbit anti‐mouse FITC (Dako, cat. no. F0261) 4′,6′−diamidino‐2‐phenylindole (DAPI; Research Organics, cat. no. 2039D) DMEM/F12 agar medium (see ) Vectastain ABC‐HRP kit, peroxidase, mouse IgG (Vector laboratories, cat. no. P‐4002) Biotinylated anti‐mouse secondary antibody (from Vectastain ABC‐HRP kit) DAB substrate kit, peroxidase (HRP), with nickel (Vector Laboratories, cat. no. SK‐4100) Avicel PH‐101 (Sigma‐Aldrich, cat. no. 11365‐1KG) Tissue culture plates: 6‐, 12‐, and 96‐well cell culture plates (Greiner Bio‐One, cat. no. 657160, 665180, and 655180, respectively) Polypropylene sterile conical tubes, 15‐ and 50‐ml (Greiner Bio‐One, cat. no. 188261 and 227270, respectively) Serological pipette, 5, 10, and 25 ml (Greiner Bio‐One, cat. no. 606180, 607180, and 760180, respectively) Universal pipette tips, 20, 200, and 1000 μl (VWR, cat. no. 76322‐134, 76322‐150, and 16466‐008, respectively) Incubator (PHCbi, model cat. no. MCO‐170AICUVDL) Fluorescence microscope

    Generated:

    Article Title: Generation of Recombinant SARS‐CoV‐2 Using a Bacterial Artificial Chromosome
    Article Snippet: The SARS‐CoV‐2 USA‐WA1/2020 sequence to generate the rSARS‐CoV‐2 is available from Genbank (accession no. MN985325) Post‐infection medium for Vero E6 cells: Dulbecco's Modified Eagle Medium (DMEM; Corning, cat. no. 15‐013‐CV) containing 2% fetal bovine serum (FBS; Avantor Seradigm, cat. no. 1500‐500) and 1× penicillin‐streptomycin‐ l ‐glutamine (PSG; Corning, cat. no. 30‐009‐CI) Phosphate‐buffered saline (PBS; see ) 10% neutral buffered formalin solution (Sigma‐Aldrich, cat. no. HT501128–4 liters) Permeabilization solution: 0.5% (v/v) Triton X‐100 (Sigma‐Aldrich, cat. no. X100−500 ML) in 1× PBS (see ) Blocking solution: 2.5% bovine serum albumin (BSA; Sigma‐Aldrich, cat. no. A9647) in 1× PBS (see ) Mouse anti‐SARS NP MAb 1C7 cross‐reactive with SARS‐CoV‐2 (Millipore Sigma, cat. no. ZMS1075) was generated at the Center for Therapeutic Antibody Development at the Icahn School of Medicine at Mount Sinai (ISMMS) and kindly provided by Dr. Thomas Moran Polyclonal rabbit anti‐mouse FITC (Dako, cat. no. F0261) 4′,6′−diamidino‐2‐phenylindole (DAPI; Research Organics, cat. no. 2039D) DMEM/F12 agar medium (see ) Vectastain ABC‐HRP kit, peroxidase, mouse IgG (Vector laboratories, cat. no. P‐4002) Biotinylated anti‐mouse secondary antibody (from Vectastain ABC‐HRP kit) DAB substrate kit, peroxidase (HRP), with nickel (Vector Laboratories, cat. no. SK‐4100) Avicel PH‐101 (Sigma‐Aldrich, cat. no. 11365‐1KG) Tissue culture plates: 6‐, 12‐, and 96‐well cell culture plates (Greiner Bio‐One, cat. no. 657160, 665180, and 655180, respectively) Polypropylene sterile conical tubes, 15‐ and 50‐ml (Greiner Bio‐One, cat. no. 188261 and 227270, respectively) Serological pipette, 5, 10, and 25 ml (Greiner Bio‐One, cat. no. 606180, 607180, and 760180, respectively) Universal pipette tips, 20, 200, and 1000 μl (VWR, cat. no. 76322‐134, 76322‐150, and 16466‐008, respectively) Incubator (PHCbi, model cat. no. MCO‐170AICUVDL) Fluorescence microscope

    Cell Culture:

    Article Title: Generation of Recombinant SARS‐CoV‐2 Using a Bacterial Artificial Chromosome
    Article Snippet: The SARS‐CoV‐2 USA‐WA1/2020 sequence to generate the rSARS‐CoV‐2 is available from Genbank (accession no. MN985325) Post‐infection medium for Vero E6 cells: Dulbecco's Modified Eagle Medium (DMEM; Corning, cat. no. 15‐013‐CV) containing 2% fetal bovine serum (FBS; Avantor Seradigm, cat. no. 1500‐500) and 1× penicillin‐streptomycin‐ l ‐glutamine (PSG; Corning, cat. no. 30‐009‐CI) Phosphate‐buffered saline (PBS; see ) 10% neutral buffered formalin solution (Sigma‐Aldrich, cat. no. HT501128–4 liters) Permeabilization solution: 0.5% (v/v) Triton X‐100 (Sigma‐Aldrich, cat. no. X100−500 ML) in 1× PBS (see ) Blocking solution: 2.5% bovine serum albumin (BSA; Sigma‐Aldrich, cat. no. A9647) in 1× PBS (see ) Mouse anti‐SARS NP MAb 1C7 cross‐reactive with SARS‐CoV‐2 (Millipore Sigma, cat. no. ZMS1075) was generated at the Center for Therapeutic Antibody Development at the Icahn School of Medicine at Mount Sinai (ISMMS) and kindly provided by Dr. Thomas Moran Polyclonal rabbit anti‐mouse FITC (Dako, cat. no. F0261) 4′,6′−diamidino‐2‐phenylindole (DAPI; Research Organics, cat. no. 2039D) DMEM/F12 agar medium (see ) Vectastain ABC‐HRP kit, peroxidase, mouse IgG (Vector laboratories, cat. no. P‐4002) Biotinylated anti‐mouse secondary antibody (from Vectastain ABC‐HRP kit) DAB substrate kit, peroxidase (HRP), with nickel (Vector Laboratories, cat. no. SK‐4100) Avicel PH‐101 (Sigma‐Aldrich, cat. no. 11365‐1KG) Tissue culture plates: 6‐, 12‐, and 96‐well cell culture plates (Greiner Bio‐One, cat. no. 657160, 665180, and 655180, respectively) Polypropylene sterile conical tubes, 15‐ and 50‐ml (Greiner Bio‐One, cat. no. 188261 and 227270, respectively) Serological pipette, 5, 10, and 25 ml (Greiner Bio‐One, cat. no. 606180, 607180, and 760180, respectively) Universal pipette tips, 20, 200, and 1000 μl (VWR, cat. no. 76322‐134, 76322‐150, and 16466‐008, respectively) Incubator (PHCbi, model cat. no. MCO‐170AICUVDL) Fluorescence microscope

    Sterility:

    Article Title: Generation of Recombinant SARS‐CoV‐2 Using a Bacterial Artificial Chromosome
    Article Snippet: The SARS‐CoV‐2 USA‐WA1/2020 sequence to generate the rSARS‐CoV‐2 is available from Genbank (accession no. MN985325) Post‐infection medium for Vero E6 cells: Dulbecco's Modified Eagle Medium (DMEM; Corning, cat. no. 15‐013‐CV) containing 2% fetal bovine serum (FBS; Avantor Seradigm, cat. no. 1500‐500) and 1× penicillin‐streptomycin‐ l ‐glutamine (PSG; Corning, cat. no. 30‐009‐CI) Phosphate‐buffered saline (PBS; see ) 10% neutral buffered formalin solution (Sigma‐Aldrich, cat. no. HT501128–4 liters) Permeabilization solution: 0.5% (v/v) Triton X‐100 (Sigma‐Aldrich, cat. no. X100−500 ML) in 1× PBS (see ) Blocking solution: 2.5% bovine serum albumin (BSA; Sigma‐Aldrich, cat. no. A9647) in 1× PBS (see ) Mouse anti‐SARS NP MAb 1C7 cross‐reactive with SARS‐CoV‐2 (Millipore Sigma, cat. no. ZMS1075) was generated at the Center for Therapeutic Antibody Development at the Icahn School of Medicine at Mount Sinai (ISMMS) and kindly provided by Dr. Thomas Moran Polyclonal rabbit anti‐mouse FITC (Dako, cat. no. F0261) 4′,6′−diamidino‐2‐phenylindole (DAPI; Research Organics, cat. no. 2039D) DMEM/F12 agar medium (see ) Vectastain ABC‐HRP kit, peroxidase, mouse IgG (Vector laboratories, cat. no. P‐4002) Biotinylated anti‐mouse secondary antibody (from Vectastain ABC‐HRP kit) DAB substrate kit, peroxidase (HRP), with nickel (Vector Laboratories, cat. no. SK‐4100) Avicel PH‐101 (Sigma‐Aldrich, cat. no. 11365‐1KG) Tissue culture plates: 6‐, 12‐, and 96‐well cell culture plates (Greiner Bio‐One, cat. no. 657160, 665180, and 655180, respectively) Polypropylene sterile conical tubes, 15‐ and 50‐ml (Greiner Bio‐One, cat. no. 188261 and 227270, respectively) Serological pipette, 5, 10, and 25 ml (Greiner Bio‐One, cat. no. 606180, 607180, and 760180, respectively) Universal pipette tips, 20, 200, and 1000 μl (VWR, cat. no. 76322‐134, 76322‐150, and 16466‐008, respectively) Incubator (PHCbi, model cat. no. MCO‐170AICUVDL) Fluorescence microscope

    Transferring:

    Article Title: Generation of Recombinant SARS‐CoV‐2 Using a Bacterial Artificial Chromosome
    Article Snippet: The SARS‐CoV‐2 USA‐WA1/2020 sequence to generate the rSARS‐CoV‐2 is available from Genbank (accession no. MN985325) Post‐infection medium for Vero E6 cells: Dulbecco's Modified Eagle Medium (DMEM; Corning, cat. no. 15‐013‐CV) containing 2% fetal bovine serum (FBS; Avantor Seradigm, cat. no. 1500‐500) and 1× penicillin‐streptomycin‐ l ‐glutamine (PSG; Corning, cat. no. 30‐009‐CI) Phosphate‐buffered saline (PBS; see ) 10% neutral buffered formalin solution (Sigma‐Aldrich, cat. no. HT501128–4 liters) Permeabilization solution: 0.5% (v/v) Triton X‐100 (Sigma‐Aldrich, cat. no. X100−500 ML) in 1× PBS (see ) Blocking solution: 2.5% bovine serum albumin (BSA; Sigma‐Aldrich, cat. no. A9647) in 1× PBS (see ) Mouse anti‐SARS NP MAb 1C7 cross‐reactive with SARS‐CoV‐2 (Millipore Sigma, cat. no. ZMS1075) was generated at the Center for Therapeutic Antibody Development at the Icahn School of Medicine at Mount Sinai (ISMMS) and kindly provided by Dr. Thomas Moran Polyclonal rabbit anti‐mouse FITC (Dako, cat. no. F0261) 4′,6′−diamidino‐2‐phenylindole (DAPI; Research Organics, cat. no. 2039D) DMEM/F12 agar medium (see ) Vectastain ABC‐HRP kit, peroxidase, mouse IgG (Vector laboratories, cat. no. P‐4002) Biotinylated anti‐mouse secondary antibody (from Vectastain ABC‐HRP kit) DAB substrate kit, peroxidase (HRP), with nickel (Vector Laboratories, cat. no. SK‐4100) Avicel PH‐101 (Sigma‐Aldrich, cat. no. 11365‐1KG) Tissue culture plates: 6‐, 12‐, and 96‐well cell culture plates (Greiner Bio‐One, cat. no. 657160, 665180, and 655180, respectively) Polypropylene sterile conical tubes, 15‐ and 50‐ml (Greiner Bio‐One, cat. no. 188261 and 227270, respectively) Serological pipette, 5, 10, and 25 ml (Greiner Bio‐One, cat. no. 606180, 607180, and 760180, respectively) Universal pipette tips, 20, 200, and 1000 μl (VWR, cat. no. 76322‐134, 76322‐150, and 16466‐008, respectively) Incubator (PHCbi, model cat. no. MCO‐170AICUVDL) Fluorescence microscope

    Fluorescence:

    Article Title: Generation of Recombinant SARS‐CoV‐2 Using a Bacterial Artificial Chromosome
    Article Snippet: The SARS‐CoV‐2 USA‐WA1/2020 sequence to generate the rSARS‐CoV‐2 is available from Genbank (accession no. MN985325) Post‐infection medium for Vero E6 cells: Dulbecco's Modified Eagle Medium (DMEM; Corning, cat. no. 15‐013‐CV) containing 2% fetal bovine serum (FBS; Avantor Seradigm, cat. no. 1500‐500) and 1× penicillin‐streptomycin‐ l ‐glutamine (PSG; Corning, cat. no. 30‐009‐CI) Phosphate‐buffered saline (PBS; see ) 10% neutral buffered formalin solution (Sigma‐Aldrich, cat. no. HT501128–4 liters) Permeabilization solution: 0.5% (v/v) Triton X‐100 (Sigma‐Aldrich, cat. no. X100−500 ML) in 1× PBS (see ) Blocking solution: 2.5% bovine serum albumin (BSA; Sigma‐Aldrich, cat. no. A9647) in 1× PBS (see ) Mouse anti‐SARS NP MAb 1C7 cross‐reactive with SARS‐CoV‐2 (Millipore Sigma, cat. no. ZMS1075) was generated at the Center for Therapeutic Antibody Development at the Icahn School of Medicine at Mount Sinai (ISMMS) and kindly provided by Dr. Thomas Moran Polyclonal rabbit anti‐mouse FITC (Dako, cat. no. F0261) 4′,6′−diamidino‐2‐phenylindole (DAPI; Research Organics, cat. no. 2039D) DMEM/F12 agar medium (see ) Vectastain ABC‐HRP kit, peroxidase, mouse IgG (Vector laboratories, cat. no. P‐4002) Biotinylated anti‐mouse secondary antibody (from Vectastain ABC‐HRP kit) DAB substrate kit, peroxidase (HRP), with nickel (Vector Laboratories, cat. no. SK‐4100) Avicel PH‐101 (Sigma‐Aldrich, cat. no. 11365‐1KG) Tissue culture plates: 6‐, 12‐, and 96‐well cell culture plates (Greiner Bio‐One, cat. no. 657160, 665180, and 655180, respectively) Polypropylene sterile conical tubes, 15‐ and 50‐ml (Greiner Bio‐One, cat. no. 188261 and 227270, respectively) Serological pipette, 5, 10, and 25 ml (Greiner Bio‐One, cat. no. 606180, 607180, and 760180, respectively) Universal pipette tips, 20, 200, and 1000 μl (VWR, cat. no. 76322‐134, 76322‐150, and 16466‐008, respectively) Incubator (PHCbi, model cat. no. MCO‐170AICUVDL) Fluorescence microscope

    Microscopy:

    Article Title: Generation of Recombinant SARS‐CoV‐2 Using a Bacterial Artificial Chromosome
    Article Snippet: The SARS‐CoV‐2 USA‐WA1/2020 sequence to generate the rSARS‐CoV‐2 is available from Genbank (accession no. MN985325) Post‐infection medium for Vero E6 cells: Dulbecco's Modified Eagle Medium (DMEM; Corning, cat. no. 15‐013‐CV) containing 2% fetal bovine serum (FBS; Avantor Seradigm, cat. no. 1500‐500) and 1× penicillin‐streptomycin‐ l ‐glutamine (PSG; Corning, cat. no. 30‐009‐CI) Phosphate‐buffered saline (PBS; see ) 10% neutral buffered formalin solution (Sigma‐Aldrich, cat. no. HT501128–4 liters) Permeabilization solution: 0.5% (v/v) Triton X‐100 (Sigma‐Aldrich, cat. no. X100−500 ML) in 1× PBS (see ) Blocking solution: 2.5% bovine serum albumin (BSA; Sigma‐Aldrich, cat. no. A9647) in 1× PBS (see ) Mouse anti‐SARS NP MAb 1C7 cross‐reactive with SARS‐CoV‐2 (Millipore Sigma, cat. no. ZMS1075) was generated at the Center for Therapeutic Antibody Development at the Icahn School of Medicine at Mount Sinai (ISMMS) and kindly provided by Dr. Thomas Moran Polyclonal rabbit anti‐mouse FITC (Dako, cat. no. F0261) 4′,6′−diamidino‐2‐phenylindole (DAPI; Research Organics, cat. no. 2039D) DMEM/F12 agar medium (see ) Vectastain ABC‐HRP kit, peroxidase, mouse IgG (Vector laboratories, cat. no. P‐4002) Biotinylated anti‐mouse secondary antibody (from Vectastain ABC‐HRP kit) DAB substrate kit, peroxidase (HRP), with nickel (Vector Laboratories, cat. no. SK‐4100) Avicel PH‐101 (Sigma‐Aldrich, cat. no. 11365‐1KG) Tissue culture plates: 6‐, 12‐, and 96‐well cell culture plates (Greiner Bio‐One, cat. no. 657160, 665180, and 655180, respectively) Polypropylene sterile conical tubes, 15‐ and 50‐ml (Greiner Bio‐One, cat. no. 188261 and 227270, respectively) Serological pipette, 5, 10, and 25 ml (Greiner Bio‐One, cat. no. 606180, 607180, and 760180, respectively) Universal pipette tips, 20, 200, and 1000 μl (VWR, cat. no. 76322‐134, 76322‐150, and 16466‐008, respectively) Incubator (PHCbi, model cat. no. MCO‐170AICUVDL) Fluorescence microscope



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